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KMID : 1098420060140020070
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Korean Journal of Medicinal Crop Science
2006 Volume.14 No. 2 p.70 ~ p.76
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Methanol Extract of Paeonia Japonica Root Protects Cultured Rat Cortical Neurons Against Oxidative Damage Induced by Hydrogen Peroxide
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Park Min-Su
Lee Ju-Hyun
Ban Ju-Yeon
Song Kyung-Sik
Seong Yeon-Hee
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Abstract
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Paeoniae radix has been widely used for its anti-allergic, anti-inflammatory and analgesic effects, and demonstrated to have anticonvulsant, memory enhancing and anxiolytic activities. The present study was performed to examine the protective effect of methanol extract of Paeoniae radix (PR) from Paeoniae Japonica Miyabe et Takeda (Paeoniaceae) on hydrogen peroxide (H_2O_2)-induced neurotoxicity using cultured rat cerebral cortical neuron. H_2O_2 produced a concentration-dependent reduction of neuronal viability, PR, over a concentration range of 10 to 100;{mu}g/ml showed concentration-dependent decrease of the H_2O_2(100;{mu}M)-induced neuronal cell death, as assessed by a 3-[4,5-dimethylthiazol-2-yl]-2,5-di-phenyl-tetrazolium bromide assay and the number of apoptotic nuclei, evidenced by Hoechst 33342 staining. PR (100;{mu}g/ml inhibited 100;{mu}M H_2O_2-induced elevation of the cytosolic Ca^{2+} concentration ([Ca^{2+}]_c), which was measured by a fluorescent dye, flue-4 AM. PR (50;{mu}g/ml inhibited glutamate release into medium induced by 100;{mu}M H_2O_2, which was measured by HPLC, and generation of reactive oxygen species (ROS). These results suggest that PR may mitigate the H_2O_2-induced neurotoxiciy by interfering with the increase of [Ca^{2+}]_c, and then inhibiting glutamate release and generation of ROS in cultured neurons.
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KEYWORD
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Paeoniae radix, neurotoxicity, hydrogen peroxide, neuroprotection, Cortical neurons
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